rpmB [2019-07-08 10:47:42]

ribosomal protein

locus: BSU15820

pI: 12.24

mw: 6.67 kDa

protein length: 62 aa

gene length: 189 bp

function: translation

product: ribosomal protein L28

essential: no

synonyms: yloT

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

Gene

Coordinates: 1,655,599 1,655,787

The protein

Protein family

• [SW|ribosomal protein] L28P family (according to UniProt)

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

Additional information

• the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA] [Pubmed|24335279,23700310]

Expression and Regulation

Operons

• genes: [gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|rpmB]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [Pubmed|11948165], in [regulon|stringent response|stringent response]

Biological materials

Mutant

• BKE15820 ([gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|rpmB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTCCCTCCTCACT, downstream forward: _UP4_TAACAAAATGAACGCCTGCC
• BKK15820 ([gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|rpmB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTCCCTCCTCACT, downstream forward: _UP4_TAACAAAATGAACGCCTGCC

References

• 19653700,23002217,23033921,23700310,24335279,25903689